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Contribute a "Map of the Month" and join the neuroscience revolution

At Vibraint, the integration of whole-brain data sets captured across diverse imaging modalities sits at the heart of our vision. If you have some beautiful light-sheet microscopy data of adult mouse brains, we invite you to showcase your work as our Map of the Month, featured on our homepage and within CNS-Voyager™

Contribute a Map of the Month

If you have some whole brain data acquired with LSFM that you would like to share with the world, please fill in the form and we will get back to you shortly. You can also write to us on contact@vibraint.dk if you have any questions.  

 

May 2026

Whole brain c-Fos after dosing with semaglutide

In the adult mouse brain, c-Fos is widely used as an immediate early gene marker of recent neuronal activation. Following Semaglutide treatment, c-Fos immunostaining highlights brain regions recruited by GLP-1 receptor–mediated signaling, with prominent activation in the dorsal vagal complex, parabrachial nucleus, central amygdala, and bed nucleus of the stria terminalis. As a rapid transcriptional response marker, c-Fos provides a spatial readout of neuronal populations engaged by pharmacological treatment. The attached image shows whole-brain c-Fos immunostaining in the adult mouse brain following Semaglutide treatment using a c-Fos antibody 
   
Protocol: iDISCO (link)
Antibody: c-Fos (link)
Acquisition: Blaze (light sheet microscope)

Contributed by: 
Daniel Sanchez Berenguer, Grace Houser and Kenneth Madsen
University of Copenhagen 

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Whole brain TH immunohistochemistry.    

Using whole brain TH staining to study Parkinson's disease.

This month's the map was contributed by Peter Gordon PhD from the Sainsbury Wellcome Centre for Neural Circuits and Behaviour at UCL. The map shows an adult mouse brain stained with a TH antibody from Miltenyi Biotec, scanned on a mesospim light sheet microscope
 
Protocol: iDISCO (link)
Antibody: TH (link)
Acquisition: Mesospim (light sheet microscope)
 
Contributed by: 
Peter Gordon 
Neuro Informatics Unit, UCL
 
 

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Different GLP-R populations 

In this "Map of the Month", the GLP-1R expressing neurons have been colour-coded to indicate whether they are GABAergic (cyan) or GLUTamatergic (magenta). This differentiation is significant, as GABAergic neurons are typically inhibitory, while GLUTamatergic neurons are activating. Sign up as a beta tester for our CNS-Voyager platform to explore the map in more detail. 

The map was generated by back-projecting single cell transcriptomics data from the Allen Institute onto a light sheet reference brain.

Contributed by: 
Thomas Topilko and Silas Larsen

February

January 2026

Whole brain MBP immunohistochemistry. 

The map represents an adult wild type mouse brain stained for Myelin Binding Protein (MBP) and scanned using a Blaze light sheet microscope. This brain is also the first brain to be stained, scanned and mapped at Vibraint, making it an important milestone in our start-up journey🎉

Protocol: iDISCO (link)
Antibody: MBP (link)
Acquisition: Blaze (light sheet microscope)


Contributed by: 
Sunny Dai and Thomas Topilko 
Vibraint ApS


Retrograde tracing of neurons after lumbar injection. 

3D map of GFP expression following AAVretor injections into the lumbar spinal cord. The map highlights the brain’s long range neurons that send axons centimeters down to the lumbar spinal cord. 

Protocol: iDISCO (link)
Antibody: GPF (link)
Acquisition: Blaze (light sheet microscope)
 

Contributed by: 
Grace Houser, Martin Carbo and Rune Berg 
University of Copenhagen 

December 2025

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Vibraint's LS-Journey™ Platform

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We are here to help you get started

At Vibraint, we are passionate about whole brain imaging and light sheet microscopy—it's at the heart of everything we do. Our LS-Journey™ platform provides quantitative whole brain imaging as a service, offering end-to-end solutions from tissue labelling and clearing to light-sheet microscopy and AI guided image analysis for segmentation and atlas registration. Each step can also be accessed individually to suit your research needs. 

Whether you want to conduct your study with us or need expert advice to begin independently, we are here to help you unlock the amazing potential of whole brain imaging.